Multisource Total RNA Miniprep Kit

Multisource Total RNA Miniprep Kit represents a new approach for total cellular RNA purification, which is designed to eliminate the problems associated with other spin column-type RNA kit, such as clogged columns and incomplete purification. Tissues and cells are first lysed by cell lysis buffer R-L, which also nullifies any indigenous RNase activity. Proteins and genomic DNA are then precipitated by the addition of neutralization buffer R-N to the cell lysate. After addition of isopropanol to supernatant, the total RNA is then bound to a spin/vac miniprep column for further washing and desalting. A high purity and full-length total cellular RNA is then eluted in a small volume of TE (or DEPC-treated water) and is ready for use in any downstream applications.